What is the objective lens used to locate the specimen and first focus?
Question: What is the objective lens used to locate the specimen and first focus?
In microscopy, the objective lens is the lens that is closest to the specimen being viewed, and it is used to magnify the image of the specimen. The objective lens is typically mounted on a rotating turret or nosepiece, which allows the user to easily switch between different objective lenses with different magnification powers.
To locate the specimen and first focus the microscope, the user typically begins by selecting the objective lens with the lowest magnification power (usually 4x or 5x). The user then positions the microscope stage so that the specimen is centered under the objective lens, and uses the coarse focus knob to bring the image into focus. Once the image is in focus, the user can switch to a higher magnification objective lens if needed, and use the fine focus knob to make any final adjustments to the focus.
It is important to note that the objective lens is just one part of the microscope's optical system, and that the total magnification of the image is determined by the combination of the objective lens and the eyepiece lens. In addition, the quality and clarity of the image also depend on the quality of the other optical components of the microscope, as well as the lighting and contrast of the specimen.
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