Suppose a pcr was set up to look for three target sequences simultaneously. how many different primers would be added the reaction during set-up?

Question: Suppose a pcr was set up to look for three target sequences simultaneously. how many different primers would be added the reaction during set-up?

To set up a PCR reaction for three target sequences, a total of six different primers would be added to the reaction. Specifically, there would be three sets of primers, with each set consisting of a forward and reverse primer that are complementary to the target DNA sequence. The primers are designed to flank the target sequence and serve as a starting point for the amplification of the DNA fragment. The PCR reaction would then proceed through cycles of denaturation, annealing, and extension, resulting in the amplification of the target sequences of interest.

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