What is the purpose of setting up a reaction without template dna when performing pcr?
Monday, April 03, 2023
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Question: What is the purpose of setting up a reaction without template dna when performing pcr?
The purpose of setting up a reaction without template DNA when performing PCR is to serve as a negative control to detect any contamination or errors that may occur during the PCR process. The absence of DNA in the negative control should result in no amplification, and any observed amplification can indicate contamination or other issues, such as non-specific primer binding. The negative control serves as a baseline for comparison to the experimental samples, and can ensure the reliability and accuracy of the PCR results.
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