Explain why it is needed to wash the leaf with water after it has been removed from the ethanol?

Question: Explain why it is needed to wash the leaf with water after it has been removed from the ethanol?

Washing the leaf with water after its removal from ethanol is an essential step in the process of chlorophyll extraction. Ethanol is used to dehydrate the leaf and remove chlorophyll, which is soluble in alcohol. Once the chlorophyll is extracted, the remaining pigments are not as soluble in ethanol. Washing with water helps to remove any residual ethanol, which could interfere with subsequent analyses or experiments. Additionally, it helps to rehydrate the leaf tissue, which may be necessary for further examination or experimentation.

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